› 3isg

X-ray diffraction
1.4Å resolution

Structure of the class D beta-lactamase OXA-1 in complex with doripenem

Released:
DOI: 10.2210/pdb3isg/pdb
PDB entry 3isg coloured by chain, front view.
PDB entry 3isg coloured by chain, side view.
PDB entry 3isg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The 1.4 A crystal structure of the class D beta-lactamase OXA-1 complexed with doripenem.
Schneider KD, Karpen ME, Bonomo RA, Leonard DA, Powers RA
Biochemistry 48 11840-7 (2009)
PMID: 19919101

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase OXA-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 28.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13661 (Residues: 26-276; Coverage: 100%)
Gene names: bla, oxa1
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand DRW 2 x DRW
Ligand MPD 1 x MPD
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 35.041Å b: 71.613Å c: 93.139Å
α: 90° β: 99.18° γ: 90°
R-values:
R R work R free
0.185 0.184 0.206
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Carbapenems: past, present, and future.
Papp-Wallace et al. (2011)
6 more

15 mentions without citation

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
14 more