› 3is8

X-ray diffraction
2.25Å resolution

Structure of mineralized Bfrb soaked with FeSO4 from Pseudomonas aeruginosa to 2.25A Resolution

Released:
DOI: 10.2210/pdb3is8/pdb
PDB entry 3is8 coloured by chain, front view.
PDB entry 3is8 coloured by chain, side view.
PDB entry 3is8 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural studies of bacterioferritin B from Pseudomonas aeruginosa suggest a gating mechanism for iron uptake via the ferroxidase center .
Weeratunga SK, Lovell S, Yao H, Battaile KP, Fischer CJ, Gee CE, Rivera M
Biochemistry 49 1160-75 (2010)
PMID: 20067302

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterioferritin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 158 amino acids
Theoretical weight: 18.58 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HY79 (Residues: 1-158; Coverage: 100%)
Gene names: PA3531, bfrB
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

4 bound ligands:
Ligand FE2 96 x FE2
Ligand K 6 x K
Ligand HEM 12 x HEM
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P212121
Unit cell:
a: 125.723Å b: 203.278Å c: 207.724Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.226
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Ferritins: furnishing proteins with iron.
Bradley et al. (2016)
4 more

5 mentions without citation

Structural studies of bacterioferritin B from Pseudomonas aeruginosa suggest a gating mechanism for iron uptake via the ferroxidase center .
Weeratunga et al. (2010)
4 more