PDBe 3ir7

X-ray diffraction
2.5Å resolution

Crystal structure of NarGHI mutant NarG-R94S

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Respiratory nitrate reductase 1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 512 amino acids
Theoretical weight: 58.14 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P11349 (Residues: 1-512; Coverage: 100%)
Gene names: JW1216, b1225, narH
Sequence domains:
Structure domains:
Respiratory nitrate reductase 1 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 1247 amino acids
Theoretical weight: 140.59 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P09152 (Residues: 1-1247; Coverage: 100%)
Gene names: JW1215, b1224, bisD, narC, narG
Sequence domains:
Structure domains:
Respiratory nitrate reductase 1 gamma chain Chain: C
Molecule details ›
Chain: C
Length: 225 amino acids
Theoretical weight: 25.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P11350 (Residues: 1-225; Coverage: 100%)
Gene names: JW1218, b1227, chlI, narI
Sequence domains: Nitrate reductase gamma subunit
Structure domains: Transmembrane di-heme cytochromes, Chain C

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 153.851Å b: 241.147Å c: 139.966Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.243
Expression system: Escherichia coli