› 3ihr

X-ray diffraction
2.95Å resolution

Crystal Structure of Uch37

Released:
DOI: 10.2210/pdb3ihr/pdb
PDB entry 3ihr coloured by chain, front view.
PDB entry 3ihr coloured by chain, side view.
PDB entry 3ihr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of human Uch37.
Burgie SE, Bingman CA, Soni AB, Phillips GN
Proteins 80 649-54 (2012)
PMID: 21953935

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase isozyme L5 Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5K5 (Residues: 1-329; Coverage: 100%)
Gene names: AD-019, CGI-70, UCH37, UCHL5
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FMT 2 x FMT
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 90.092Å b: 98.736Å c: 154.061Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.242
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
10 more

27 mentions without citation

Regulation of proteolysis by human deubiquitinating enzymes.
Eletr et al. (2014)
26 more