PDBe 3ihg

X-ray diffraction
2.49Å resolution

Crystal structure of a ternary complex of aklavinone-11 hydroxylase with FAD and aklavinone

Released:

Function and Biology Details

Reaction catalysed:
Aklavinone + NADPH + O(2) = epsilon-rhodomycinone + NADP(+) + H(2)O. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aklavinone 12-hydroxylase RdmE Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 535 amino acids
Theoretical weight: 57.5 KDa
Source organism: Streptomyces purpurascens
Expression system: Streptomyces lividans
UniProt:
  • Canonical: Q54530 (Residues: 1-535; Coverage: 100%)
Gene name: rdmE
Sequence domains:
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P63
Unit cell:
a: 183.035Å b: 183.035Å c: 99.639Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.204 0.255
Expression system: Streptomyces lividans