› 3iev

X-ray diffraction
1.9Å resolution

Crystal Structure of ERA in Complex with MgGNP and the 3' End of 16S rRNA

Released:
DOI: 10.2210/pdb3iev/pdb
PDB entry 3iev coloured by chain, front view.
PDB entry 3iev coloured by chain, side view.
PDB entry 3iev coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structure of ERA in complex with the 3' end of 16S rRNA: implications for ribosome biogenesis.
Tu C, Zhou X, Tropea JE, Austin BP, Waugh DS, Court DL, Ji X
Proc Natl Acad Sci U S A 106 14843-8 (2009)
PMID: 19706445

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130561 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
GTPase Era Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 34.82 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67800 (Residues: 1-301; Coverage: 100%)
Gene names: aq_1994, era, era1
Sequence domains:
Structure domains:
5'-R(P*AP*UP*CP*AP*CP*CP*UP*CP*CP*UP*UP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.69 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 119.6Å b: 71.6Å c: 43.6Å
α: 90° β: 101.5° γ: 90°
R-values:
R R work R free
0.185 0.183 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
15 more

17 mentions without citation

The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly.
Bennison et al. (2019)
16 more