PDBe 3iec

X-ray diffraction
2.2Å resolution

Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein. 
ATP + [tau protein] = ADP + [tau protein] phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase MARK2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 36.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7KZI7 (Residues: 49-363; Coverage: 40%)
Gene names: EMK1, MARK2
Sequence domains:
Structure domains:
Cytotoxicity-associated immunodominant antigen Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 125 amino acids
Theoretical weight: 13.42 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: P55980 (Residues: 885-1005; Coverage: 10%)
Gene names: HP_0547, cag26, cagA, cai

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 93.466Å b: 93.256Å c: 113.955Å
α: 90° β: 100.94° γ: 90°
R-values:
R R work R free
0.206 0.204 0.248
Expression system: Escherichia coli