› 3ia7

X-ray diffraction
1.91Å resolution

Crystal Structure of CalG4, the Calicheamicin Glycosyltransferase

Released:
DOI: 10.2210/pdb3ia7/pdb
PDB entry 3ia7 coloured by chain, front view.
PDB entry 3ia7 coloured by chain, side view.
PDB entry 3ia7 coloured by chain, top view.
Source organism: Micromonospora echinospora
Primary publication:
Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.
Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN
Proc Natl Acad Sci U S A 108 17649-54 (2011)
PMID: 21987796

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Erythromycin biosynthesis protein CIII-like C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 402 amino acids
Theoretical weight: 44.42 KDa
Source organism: Micromonospora echinospora
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KNC3 (Residues: 2-401; Coverage: 100%)
Gene name: calG4
Sequence domains: Erythromycin biosynthesis protein CIII-like, C-terminal domain
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 84.749Å b: 66.966Å c: 86.616Å
α: 90° β: 93.53° γ: 90°
R-values:
R R work R free
0.256 0.253 0.301
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Recent structures, evolution and mechanisms of glycosyltransferases.
Breton et al. (2012)
6 more

3 mentions without citation

Glycosyltransferase structural biology and its role in the design of catalysts for glycosylation.
Chang et al. (2011)
2 more