› 3i5a

X-ray diffraction
2.8Å resolution

Crystal structure of full-length WpsR from Pseudomonas syringae

Released:
DOI: 10.2210/pdb3i5a/pdb
PDB entry 3i5a coloured by chain, front view.
PDB entry 3i5a coloured by chain, side view.
PDB entry 3i5a coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Primary publication:
Determinants for the activation and autoinhibition of the diguanylate cyclase response regulator WspR.
De N, Navarro MV, Raghavan RV, Sondermann H
J Mol Biol 393 619-33 (2009)
PMID: 19695263

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183425 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 36.95 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli
UniProt:
  • Canonical: Q886S7 (Residues: 1-334; Coverage: 100%)
Gene name: PSPTO_1499
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand C2E 2 x C2E
Ligand SR 1 x SR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C222
Unit cell:
a: 111.7Å b: 181.2Å c: 39.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.238 0.266
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
20 more

6 mentions without citation

Diversity of Cyclic Di-GMP-Binding Proteins and Mechanisms.
Chou et al. (2016)
5 more