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X-ray investigation of gene-engineered human insulin crystallized from a solution containing polysialic acid.

Timofeev VI, Chuprov-Netochin RN, Samigina VR, Bezuglov VV, Miroshnikov KA, Kuranova IP
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 259-63 (2010)
Cited: 15 times
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Abstract

Attempts to crystallize the noncovalent complex of recombinant human insulin with polysialic acid were carried out under normal and microgravity conditions. Both crystal types belonged to the same space group, I2(1)3, with unit-cell parameters a = b = c = 77.365 A, alpha = beta = gamma = 90.00 degrees. The reported space group and unit-cell parameters are almost identical to those of cubic insulin reported in the PDB. The results of X-ray studies confirmed that the crystals obtained were cubic insulin crystals and that they contained no polysialic acid or its fragments. Electron-density maps were calculated using X-ray diffraction sets from earth-grown and microgravity-grown crystals and the three-dimensional structure of the insulin molecule was determined and refined. The conformation and secondary-structural elements of the insulin molecule in different crystal forms were compared.

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  2. Oligomerization of Peptides LVEALYL and RGFFYT and Their Binding Affinity to Insulin. Chiang HL, Ngo ST, Chen CJ, Hu CK, Li MS. PLoS ONE 8 e65358 (2013)
  3. Examining protein surface structure in highly conserved sequence variants with mass spectrometry. Tao Y, Julian RR. Biochemistry 51 1796-1802 (2012)
  4. Identifying signatures of proteolytic stability and monomeric propensity in O-glycosylated insulin using molecular simulation. Hsu WT, Ramirez DA, Sammakia T, Tan Z, Shirts MR. J Comput Aided Mol Des 36 313-328 (2022)
  5. Insulin in motion: The A6-A11 disulfide bond allosterically modulates structural transitions required for insulin activity. van Lierop B, Ong SC, Belgi A, Delaine C, Andrikopoulos S, Haworth NL, Menting JG, Lawrence MC, Robinson AJ, Forbes BE. Sci Rep 7 17239 (2017)
  6. X-ray investigation of gene-engineered human insulin crystallized from a solution containing polysialic acid. Timofeev VI, Chuprov-Netochin RN, Samigina VR, Bezuglov VV, Miroshnikov KA, Kuranova IP. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 259-263 (2010)
  7. General method to stabilize mesophilic proteins in hyperthermal water. Xin X, Xu Y, Shi H, Liu X. iScience 24 102503 (2021)
  8. The T2 structure of polycrystalline cubic human insulin. Triandafillidis DP, Karavassili F, Spiliopoulou M, Valmas A, Athanasiadou M, Nikolaras G, Fili S, Kontou P, Bowler MW, Chasapis CT, Von Dreele RB, Fitch AN, Margiolaki I. Acta Crystallogr D Struct Biol 79 374-386 (2023)


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  1. A minimized human insulin-receptor-binding motif revealed in a Conus geographus venom insulin. Menting JG, Gajewiak J, MacRaild CA, Chou DH, Disotuar MM, Smith NA, Miller C, Erchegyi J, Rivier JE, Olivera BM, Forbes BE, Smith BJ, Norton RS, Safavi-Hemami H, Lawrence MC. Nat. Struct. Mol. Biol. 23 916-920 (2016)
  2. Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling. Kotowska AM, Trindade GF, Mendes PM, Williams PM, Aylott JW, Shard AG, Alexander MR, Scurr DJ. Nat Commun 11 5832 (2020)
  3. Mechanics of controlled release of insulin entrapped in polyacrylic acid gels via variable electrical stimuli. Mallawarachchi S, Mahadevan A, Gejji V, Fernando S. Drug Deliv Transl Res 9 783-794 (2019)
  4. [Solid state isotope hydrogen exchange for deuterium and tritium in human gene-engineered insulin]. Zolotarev YA, Dadayan AK, Kozik VS, Gasanov EV, Nazimov IV, Ziganshin RKh, Vaskovsky BV, Murashov AN, Ksenofontov AL, Haribin ON, Nikolaev EN, Myasoedov NF. Bioorg Khim 40 31-41 (2014)
  5. Crystallization of Enantiomerically Pure Proteins from Quasi-Racemic Mixtures: Structure Determination by X-Ray Diffraction of Isotope-Labeled Ester Insulin and Human Insulin. Mandal K, Dhayalan B, Avital-Shmilovici M, Tokmakoff A, Kent SB. Chembiochem 17 421-425 (2016)
  6. Amyloidogenesis via interfacial shear in a containerless biochemical reactor aboard the International Space Station. McMackin P, Adam J, Griffin S, Hirsa A. NPJ Microgravity 8 41 (2022)
  7. Protein complex prediction using Rosetta, AlphaFold, and mass spectrometry covalent labeling. Drake ZC, Seffernick JT, Lindert S. Nat Commun 13 7846 (2022)


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