› 3hwr

X-ray diffraction
2.15Å resolution

Crystal structure of PanE/ApbA family ketopantoate reductase (YP_299159.1) from Ralstonia eutropha JMP134 at 2.15 A resolution

Released:
DOI: 10.2210/pdb3hwr/pdb
PDB entry 3hwr coloured by chain, front view.
PDB entry 3hwr coloured by chain, side view.
PDB entry 3hwr coloured by chain, top view.
Source organism: Cupriavidus pinatubonensis JMP134
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydropantoate 2-reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 318 amino acids
Theoretical weight: 34.41 KDa
Source organism: Cupriavidus pinatubonensis JMP134
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46RB9 (Residues: 1-299; Coverage: 100%)
Gene name: Reut_B4967
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
2 bound ligands:
Ligand BCN 2 x BCN
Ligand MRD 2 x MRD
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2, SSRL BEAMLINE BL9-2
Spacegroup: P61
Unit cell:
a: 116.21Å b: 116.21Å c: 95.604Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.183 0.226
Expression system: Escherichia coli

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