› 3hu3

X-ray diffraction
2.2Å resolution

Structure of p97 N-D1 R155H mutant in complex with ATPgS

Released:
DOI: 10.2210/pdb3hu3/pdb
PDB entry 3hu3 coloured by chain, front view.
PDB entry 3hu3 coloured by chain, side view.
PDB entry 3hu3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel ATP-dependent conformation in p97 N-D1 fragment revealed by crystal structures of disease-related mutants.
Tang WK, Li D, Li CC, Esser L, Dai R, Guo L, Xia D
EMBO J 29 2217-29 (2010)
PMID: 20512113

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transitional endoplasmic reticulum ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 54.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55072 (Residues: 1-481; Coverage: 60%)
Gene names: HEL-220, HEL-S-70, VCP
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AGS 2 x AGS
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R3
Unit cell:
a: 134.206Å b: 134.206Å c: 182.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.193
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Emerging functions of the VCP/p97 AAA-ATPase in the ubiquitin system.
Meyer et al. (2012)
21 more

8 mentions without citation

Structure and function of the AAA+ ATPase p97/Cdc48p.
Xia et al. (2016)
7 more