PDBe 3hqg

X-ray diffraction
2.6Å resolution

Crystal structure of restriction endonuclease EcoRII catalytic C-terminal domain in complex with cognate DNA

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Type-2 restriction enzyme EcoRII Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 25.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P14633 (Residues: 183-404; Coverage: 55%)
Gene name: ecoRIIR
Sequence domains: EcoRII C terminal
5'-D(*TP*AP*GP*CP*CP*TP*GP*GP*TP*CP*GP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*TP*CP*GP*AP*CP*CP*AP*GP*GP*CP*TP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21212
Unit cell:
a: 77.054Å b: 57.971Å c: 61.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.236 0.293
Expression systems:
  • Escherichia coli
  • Not provided