› 3ho8

X-ray diffraction
2.9Å resolution

Crystal Structure of S. aureus Pyruvate Carboxylase in complex with Coenzyme A

Released:
DOI: 10.2210/pdb3ho8/pdb
PDB entry 3ho8 coloured by chain, front view.
PDB entry 3ho8 coloured by chain, side view.
PDB entry 3ho8 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
A symmetrical tetramer for S. aureus pyruvate carboxylase in complex with coenzyme A.
Yu LP, Xiang S, Lasso G, Gil D, Valle M, Tong L
Structure 17 823-32 (2009)
PMID: 19523900

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate carboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1150 amino acids
Theoretical weight: 128.71 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3JRU9 (Residues: 1-1150; Coverage: 100%)
Gene names: SAV1114, pycA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand BTI 2 x BTI
2 bound ligands:
Ligand COA 4 x COA
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 96.586Å b: 164.466Å c: 373.346Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.264 0.328
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure and function of biotin-dependent carboxylases.
Tong L. (2013)
6 more

2 mentions without citation

c-di-AMP modulates Listeria monocytogenes central metabolism to regulate growth, antibiotic resistance and osmoregulation.
Whiteley et al. (2017)
1 more