PDBe 3hmy

X-ray diffraction
2Å resolution

Crystal structure of HCR/T complexed with GT2

Released:
Source organism: Clostridium tetani
Primary publication:
Gangliosides as high affinity receptors for tetanus neurotoxin.
J. Biol. Chem. 284 26569-77 (2009)
PMID: 19602728

Function and Biology Details

Reaction catalysed:
Hydrolysis of -Gln(76)-|-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetanus toxin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 51.57 KDa
Source organism: Clostridium tetani
Expression system: Escherichia coli
UniProt:
  • Canonical: P04958 (Residues: 866-1315; Coverage: 34%)
Gene names: CTC_p60, tetX
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 66.96Å b: 72.69Å c: 119.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.237
Expression system: Escherichia coli