PDBe 3hkw

X-ray diffraction
1.55Å resolution

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate. 
NTP + H(2)O = NDP + phosphate. 
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'. 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 581 amino acids
Theoretical weight: 64.32 KDa
Source organism: Hepatitis C virus subtype 1a
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26664 (Residues: 2421-2990; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 53.525Å b: 271.306Å c: 61.007Å
α: 90° β: 98.82° γ: 90°
R-values:
R R work R free
0.17 0.17 0.189
Expression system: Escherichia coli BL21(DE3)