PDBe 3hi6

X-ray diffraction
2.3Å resolution

Crystal structure of intermediate affinity I domain of integrin LFA-1 with the Fab fragment of its antibody AL-57

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Integrin alpha-L Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20701 (Residues: 153-332; Coverage: 16%)
Gene names: CD11A, ITGAL
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain
Heavy chain of Fab fragment of AL-57 against alpha L I domain Chains: H, X
Molecule details ›
Chains: H, X
Length: 220 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
light chain of Fab fragment of AL-57 against alpha L I domain Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 212 amino acids
Theoretical weight: 23.02 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 133.78Å b: 133.78Å c: 161.05Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.17 0.226
Expression systems:
  • Escherichia coli
  • Cricetulus griseus