PDBe 3hgk

X-ray diffraction
3.3Å resolution

crystal structure of effect protein AvrptoB complexed with kinase Pto

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pto kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 327 amino acids
Theoretical weight: 37.33 KDa
Source organism: Solanum pimpinellifolium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q40234 (Residues: 1-321; Coverage: 100%)
Gene name: Pto
Sequence domains: Protein tyrosine kinase
Structure domains:
Effector protein HopAB2 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 85 amino acids
Theoretical weight: 9.43 KDa
Source organism: Pseudomonas syringae pv. tomato
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RSY1 (Residues: 121-205; Coverage: 15%)
Gene names: PSPTO_3087, avrPtoB, hopAB2

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 61.07Å b: 104.47Å c: 298.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.318 0.317 0.33
Expression system: Escherichia coli