PDBe 3hdd

X-ray diffraction
2.2Å resolution

ENGRAILED HOMEODOMAIN DNA COMPLEX

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Segmentation polarity homeobox protein engrailed Chains: A, B
Molecule details ›
Chains: A, B
Length: 60 amino acids
Theoretical weight: 7.32 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P02836 (Residues: 454-513; Coverage: 11%)
Gene names: CG9015, en
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
5'-D(*AP*TP*TP*AP*GP*GP*TP*AP*AP*TP*TP*AP*CP*AP*TP*GP*GP*CP*AP*AP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*TP*TP*TP*TP*GP*CP*CP*AP*TP*GP*TP*AP*AP*TP*TP*AP*CP*CP*TP*AP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.39 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 130.62Å b: 45.52Å c: 72.93Å
α: 90° β: 118.32° γ: 90°
R-values:
R R work R free
0.204 0.204 0.232
Expression systems:
  • Escherichia coli
  • Not provided