› 3hb7

X-ray diffraction
2.3Å resolution

The Crystal Structure of an Isochorismatase-like Hydrolase from Alkaliphilus metalliredigens to 2.3A

Released:
DOI: 10.2210/pdb3hb7/pdb
PDB entry 3hb7 coloured by chain, front view.
PDB entry 3hb7 coloured by chain, side view.
PDB entry 3hb7 coloured by chain, top view.
Source organism: Alkaliphilus metalliredigens QYMF
Entry authors: Stein AJ, Xu X, Cui H, Ng J, Edwards A, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isochorismatase-like domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 204 amino acids
Theoretical weight: 23.34 KDa
Source organism: Alkaliphilus metalliredigens QYMF
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6TWT6 (Residues: 1-200; Coverage: 100%)
Gene name: Amet_4583
Sequence domains: Isochorismatase family
Structure domains: Isochorismatase-like

Ligands and Environments

2 bound ligands:
Ligand NH4 6 x NH4
Ligand NA 5 x NA
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 85.651Å b: 105.425Å c: 106.267Å
α: 90° β: 103.9° γ: 90°
R-values:
R R work R free
0.198 0.196 0.238
Expression system: Escherichia coli BL21(DE3)

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