› 3h5c

X-ray diffraction
3.26Å resolution

X-Ray Structure of Protein Z-Protein Z Inhibitor Complex

Released:
DOI: 10.2210/pdb3h5c/pdb
PDB entry 3h5c coloured by chain, front view.
PDB entry 3h5c coloured by chain, side view.
PDB entry 3h5c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Basis for the specificity and activation of the serpin protein Z-dependent proteinase inhibitor (ZPI) as an inhibitor of membrane-associated factor Xa.
Huang X, Dementiev A, Olson ST, Gettins PG
J. Biol. Chem. 285 20399-409 (2010)
PMID: 20427285

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149821 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Z-dependent protease inhibitor Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 48.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UK55 (Residues: 22-444; Coverage: 100%)
Gene names: SERPINA10, UNQ707/PRO1358, ZPI
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Vitamin K-dependent protein Z Chain: B
Molecule details ›
Chain: B
Length: 317 amino acids
Theoretical weight: 35.21 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P22891 (Residues: 84-400; Coverage: 84%)
Gene name: PROZ
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 6 x NAG
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6522
Unit cell:
a: 119.934Å b: 119.934Å c: 347.672Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.201 0.269
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

Inhibitory serpins. New insights into their folding, polymerization, regulation and clearance.
Gettins et al. (2016)
4 more

11 mentions without citation

Basis for the specificity and activation of the serpin protein Z-dependent proteinase inhibitor (ZPI) as an inhibitor of membrane-associated factor Xa.
Huang et al. (2010)
10 more