3h3w Citations

The structure of gene product 6 of bacteriophage T4, the hinge-pin of the baseplate.

Structure 17 800-8 (2009)
Related entries: 3h2t, 3h3y

Cited: 16 times
EuropePMC logo PMID: 19523898

Abstract

The baseplate of bacteriophage T4 is a multicomponent protein complex, which controls phage attachment to the host. It assembles from six wedges and a central hub. During infection the baseplate undergoes a large conformational change from a dome-shaped to a flat, star-shaped structure. We report the crystal structure of the C-terminal half of gene product (gp) 6 and investigate its motion with respect to the other proteins during the baseplate rearrangement. Six gp6 dimers interdigitate, forming a ring that maintains the integrity of the baseplate in both conformations. One baseplate wedge contains an N-terminal dimer of gp6, whereas neighboring wedges are tied together through the C-terminal dimer of gp6. The dimeric interactions are preserved throughout the rearrangement of the baseplate. However, the hinge angle between the N- and C-terminal parts of gp6 changes by approximately 15 degrees , accounting for a 10 A radial increase in the diameter of the gp6 ring.

Reviews citing this publication (4)

  1. Structure and function of bacteriophage T4. Yap ML, Rossmann MG. Future Microbiol 9 1319-1327 (2014)
  2. Structure of viruses: a short history. Rossmann MG. Q. Rev. Biophys. 46 133-180 (2013)
  3. Bacteriophage assembly. Aksyuk AA, Rossmann MG. Viruses 3 172-203 (2011)
  4. Morphogenesis of the T4 tail and tail fibers. Leiman PG, Arisaka F, van Raaij MJ, Kostyuchenko VA, Aksyuk AA, Kanamaru S, Rossmann MG. Virol. J. 7 355 (2010)

Articles citing this publication (12)

  1. EMDataBank.org: unified data resource for CryoEM. Lawson CL, Baker ML, Best C, Bi C, Dougherty M, Feng P, van Ginkel G, Devkota B, Lagerstedt I, Ludtke SJ, Newman RH, Oldfield TJ, Rees I, Sahni G, Sala R, Velankar S, Warren J, Westbrook JD, Henrick K, Kleywegt GJ, Berman HM, Chiu W. Nucleic Acids Res. 39 D456-64 (2011)
  2. Structure of the type VI secretion system contractile sheath. Kudryashev M, Wang RY, Brackmann M, Scherer S, Maier T, Baker D, DiMaio F, Stahlberg H, Egelman EH, Basler M. Cell 160 952-962 (2015)
  3. Structure of the bacteriophage T4 long tail fiber receptor-binding tip. Bartual SG, Otero JM, Garcia-Doval C, Llamas-Saiz AL, Kahn R, Fox GC, van Raaij MJ. Proc. Natl. Acad. Sci. U.S.A. 107 20287-20292 (2010)
  4. Visualizing the structural changes of bacteriophage Epsilon15 and its Salmonella host during infection. Chang JT, Schmid MF, Haase-Pettingell C, Weigele PR, King JA, Chiu W. J. Mol. Biol. 402 731-740 (2010)
  5. The Type VI Secretion TssEFGK-VgrG Phage-Like Baseplate Is Recruited to the TssJLM Membrane Complex via Multiple Contacts and Serves As Assembly Platform for Tail Tube/Sheath Polymerization. Brunet YR, Zoued A, Boyer F, Douzi B, Cascales E. PLoS Genet. 11 e1005545 (2015)
  6. The baseplate wedges of bacteriophage T4 spontaneously assemble into hubless baseplate-like structure in vitro. Yap ML, Mio K, Leiman PG, Kanamaru S, Arisaka F. J. Mol. Biol. 395 349-360 (2010)
  7. Listeria phage A511, a model for the contractile tail machineries of SPO1-related bacteriophages. Habann M, Leiman PG, Vandersteegen K, Van den Bossche A, Lavigne R, Shneider MM, Bielmann R, Eugster MR, Loessner MJ, Klumpp J. Mol. Microbiol. 92 84-99 (2014)
  8. TssA forms a gp6-like ring attached to the type VI secretion sheath. Planamente S, Salih O, Manoli E, Albesa-Jové D, Freemont PS, Filloux A. EMBO J. 35 1613-1627 (2016)
  9. Role of bacteriophage T4 baseplate in regulating assembly and infection. Yap ML, Klose T, Arisaka F, Speir JA, Veesler D, Fokine A, Rossmann MG. Proc. Natl. Acad. Sci. U.S.A. 113 2654-2659 (2016)
  10. Unified data resource for cryo-EM. Lawson CL. Meth. Enzymol. 483 73-90 (2010)
  11. Sequential assembly of the wedge of the baseplate of phage T4 in the presence and absence of gp11 as monitored by analytical ultracentrifugation. Yap ML, Mio K, Ali S, Minton A, Kanamaru S, Arisaka F. Macromol Biosci 10 808-813 (2010)
  12. Structure of the 3.3MDa, in vitro assembled, hubless bacteriophage T4 baseplate. Yap ML, Klose T, Plevka P, Aksyuk A, Zhang X, Arisaka F, Rossmann MG. J. Struct. Biol. 187 95-102 (2014)