PDBe 3h3b

X-ray diffraction
2.45Å resolution

Crystal structure of the single-chain Fv (scFv) fragment of an anti-ErbB2 antibody chA21 in complex with residues 1-192 of ErbB2 extracellular domain

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
anti-ErbB2 antibody chA21 Chains: C, D
Molecule details ›
Chains: C, D
Length: 259 amino acids
Theoretical weight: 27.56 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Receptor tyrosine-protein kinase erbB-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 21.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04626 (Residues: 23-214; Coverage: 16%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains: Receptor L domain
Structure domains: 24 nucleotide stem-loop, u2 snrnp hairpin iv. U2 a'. Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P212121
Unit cell:
a: 82.177Å b: 87.2Å c: 108.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.255
Expression systems:
  • Cricetulus griseus
  • Escherichia coli