› 3h2z

X-ray diffraction
1.9Å resolution

The crystal structure of mannitol-1-phosphate dehydrogenase from Shigella flexneri

Released:
DOI: 10.2210/pdb3h2z/pdb
PDB entry 3h2z coloured by chain, front view.
PDB entry 3h2z coloured by chain, side view.
PDB entry 3h2z coloured by chain, top view.
Source organism: Shigella flexneri 2a str. 2457T
Entry authors: Wu R, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
D-mannitol 1-phosphate + NAD(+) = D-fructose 6-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannitol-1-phosphate 5-dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 41.56 KDa
Source organism: Shigella flexneri 2a str. 2457T
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83PQ0 (Residues: 1-382; Coverage: 100%)
Gene names: S4134, SF3634, mtlD
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ACT 4 x ACT
Ligand GOL 1 x GOL
Ligand NA 5 x NA
Ligand PO4 4 x PO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I422
Unit cell:
a: 151.092Å b: 151.092Å c: 92.179Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.204
Expression system: Escherichia coli

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