PDBe 3h0r

X-ray diffraction
3Å resolution

Structure of trna-dependent amidotransferase gatcab from aquifex aeolicus

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glutamyl-tRNA(Gln) amidotransferase subunit A Chains: A, D, G, J, M, P, S, V
Molecule details ›
Chains: A, D, G, J, M, P, S, V
Length: 478 amino acids
Theoretical weight: 53.59 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66610 (Residues: 1-478; Coverage: 100%)
Gene names: aq_247, gatA
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B Chains: B, E, H, K, N, Q, T, W
Molecule details ›
Chains: B, E, H, K, N, Q, T, W
Length: 478 amino acids
Theoretical weight: 55.13 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66766 (Residues: 1-478; Coverage: 100%)
Gene names: aq_461, gatB
Sequence domains:
Structure domains: DNA polymerase; domain 1
Glutamyl-tRNA(Gln) amidotransferase subunit C Chains: C, F, I, L, O, R, U, X
Molecule details ›
Chains: C, F, I, L, O, R, U, X
Length: 94 amino acids
Theoretical weight: 11.23 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67904 (Residues: 1-94; Coverage: 100%)
Gene names: aq_2149, gatC
Sequence domains: Glu-tRNAGln amidotransferase C subunit
Structure domains: Histone, subunit A

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 127.377Å b: 130.411Å c: 153.973Å
α: 89.89° β: 90.21° γ: 89.95°
R-values:
R R work R free
0.265 0.263 0.306
Expression system: Escherichia coli