› 3h0l

X-ray diffraction
2.3Å resolution

Structure of trna-dependent amidotransferase gatcab from aquifex aeolicus

Released:
DOI: 10.2210/pdb3h0l/pdb
PDB entry 3h0l coloured by chain, front view.
PDB entry 3h0l coloured by chain, side view.
PDB entry 3h0l coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Insights into tRNA-dependent amidotransferase evolution and catalysis from the structure of the Aquifex aeolicus enzyme.
Wu J, Bu W, Sheppard K, Kitabatake M, Kwon ST, Söll D, Smith JL
J Mol Biol 391 703-16 (2009)
PMID: 19520089

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-130349 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glutamyl-tRNA(Gln) amidotransferase subunit A Chains: A, D, G, J, M, P, S, V
Molecule details ›
Chains: A, D, G, J, M, P, S, V
Length: 478 amino acids
Theoretical weight: 53.59 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66610 (Residues: 1-478; Coverage: 100%)
Gene names: aq_247, gatA
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B Chains: B, E, H, K, N, Q, T, W
Molecule details ›
Chains: B, E, H, K, N, Q, T, W
Length: 478 amino acids
Theoretical weight: 55.13 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66766 (Residues: 1-478; Coverage: 100%)
Gene names: aq_461, gatB
Sequence domains:
Glutamyl-tRNA(Gln) amidotransferase subunit C Chains: C, F, I, L, O, R, U, X
Molecule details ›
Chains: C, F, I, L, O, R, U, X
Length: 94 amino acids
Theoretical weight: 11.23 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67904 (Residues: 1-94; Coverage: 100%)
Gene names: aq_2149, gatC
Sequence domains: Glu-tRNAGln amidotransferase C subunit
Structure domains: Glu-tRNAGln amidotransferase C subunit, N-terminal domain

Ligands and Environments

4 bound ligands:
Ligand ASN 8 x ASN
Ligand ADP 8 x ADP
Ligand MG 8 x MG
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 127.482Å b: 131.012Å c: 154.668Å
α: 90.02° β: 90° γ: 89.91°
R-values:
R R work R free
0.242 0.24 0.273
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Gates of enzymes.
Gora et al. (2013)
2 more

2 mentions without citation

Insights into tRNA-dependent amidotransferase evolution and catalysis from the structure of the Aquifex aeolicus enzyme.
Wu et al. (2009)
1 more