› 3h03

X-ray diffraction
1.9Å resolution

Crystal structure of the binding domain of the AMPA subunit GluR2 bound to UBP277

Released:
DOI: 10.2210/pdb3h03/pdb
PDB entry 3h03 coloured by chain, front view.
PDB entry 3h03 coloured by chain, side view.
PDB entry 3h03 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Mechanisms of antagonism of the GluR2 AMPA receptor: structure and dynamics of the complex of two willardiine antagonists with the glutamate binding domain.
Ahmed AH, Thompson MD, Fenwick MK, Romero B, Loh AP, Jane DE, Sondermann H, Oswald RE
Biochemistry 48 3894-903 (2009)
PMID: 19284741

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B, D, G
Molecule details ›
Chains: A, B, D, G
Length: 258 amino acids
Theoretical weight: 28.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19491 (Residues: 414-527, 653-794; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand UBP 4 x UBP
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 98.953Å b: 57.719Å c: 105.461Å
α: 90° β: 114.88° γ: 90°
R-values:
R R work R free
0.221 0.221 0.258
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Glutamate receptor ion channels: structure, regulation, and function.
Traynelis et al. (2010)
5 more

4 mentions without citation

Willardiine and Its Synthetic Analogues: Biological Aspects and Implications in Peptide Chemistry of This Nucleobase Amino Acid.
Palumbo et al. (2022)
3 more