PDBe 3gzn

X-ray diffraction
3Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
NEDD8-activating enzyme E1 regulatory subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 534 amino acids
Theoretical weight: 60.32 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13564 (Residues: 1-534; Coverage: 100%)
Gene names: APPBP1, HPP1, NAE1
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain
NEDD8-activating enzyme E1 catalytic subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 463 amino acids
Theoretical weight: 51.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TBC4 (Residues: 1-463; Coverage: 100%)
Gene names: UBA3, UBE1C
Sequence domains:
Structure domains:
NEDD8 Chains: I, J
Molecule details ›
Chains: I, J
Length: 82 amino acids
Theoretical weight: 9.4 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15843 (Residues: 1-76; Coverage: 94%)
Gene name: NEDD8
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I222
Unit cell:
a: 135.03Å b: 228.715Å c: 229.307Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.287
Expression system: Spodoptera frugiperda