› 3gr9

X-ray diffraction
2.2Å resolution

Crystal structure of ColD H188K S187N

Released:
DOI: 10.2210/pdb3gr9/pdb
PDB entry 3gr9 coloured by chain, front view.
PDB entry 3gr9 coloured by chain, side view.
PDB entry 3gr9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Two site-directed mutations are required for the conversion of a sugar dehydratase into an aminotransferase.
Cook PD, Kubiak RL, Toomey DP, Holden HM
Biochemistry 48 5246-53 (2009)
PMID: 19402712

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109289 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxamine 5-phosphate-dependent dehydrase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 390 amino acids
Theoretical weight: 44.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B1B4V9 (Residues: 1-388; Coverage: 100%)
Gene name: wbdK
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AKG 6 x AKG
1 modified residue:
Ligand LLP 8 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P1
Unit cell:
a: 69.728Å b: 114.659Å c: 114.568Å
α: 78.98° β: 76.23° γ: 76.33°
R-values:
R R work R free
0.18 0.177 0.272
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
1 more

1 mention without citation

Two site-directed mutations are required for the conversion of a sugar dehydratase into an aminotransferase.
Cook et al. (2009)