PDBe 3gqi

X-ray diffraction
2.5Å resolution

Crystal Structure of activated receptor tyrosine kinase in complex with substrates

Released:

Function and Biology Details

Reactions catalysed:
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Chain: B
Molecule details ›
Chain: B
Length: 226 amino acids
Theoretical weight: 26.32 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10686 (Residues: 545-770; Coverage: 18%)
Gene name: Plcg1
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein
Fibroblast growth factor receptor 1 Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 37.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11362 (Residues: 458-774; Coverage: 40%)
Gene names: BFGFR, CEK, FGFBR, FGFR1, FLG, FLT2, HBGFR
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 133.935Å b: 64.596Å c: 94.209Å
α: 90° β: 96.5° γ: 90°
R-values:
R R work R free
0.249 0.249 0.289
Expression system: Escherichia coli