› 3gqa

X-ray diffraction
2.1Å resolution

Crystal Structure of the Bacteriophage phi29 gene product 12 N-terminal fragment in complex with cobalt ions

Released:
DOI: 10.2210/pdb3gqa/pdb
PDB entry 3gqa coloured by chain, front view.
PDB entry 3gqa coloured by chain, side view.
PDB entry 3gqa coloured by chain, top view.
Source organism: Bacillus virus phi29
Primary publication:
Crystallographic insights into the autocatalytic assembly mechanism of a bacteriophage tail spike.
Xiang Y, Leiman PG, Li L, Grimes S, Anderson DL, Rossmann MG
Mol Cell 34 375-86 (2009)
PMID: 19450535

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
gp12* Chain: A
Molecule details ›
Chain: A
Length: 609 amino acids
Theoretical weight: 64.41 KDa
Source organism: Bacillus virus phi29
Expression system: Escherichia coli
UniProt:
  • Canonical: P20345 (Residues: 89-691; Coverage: 71%)
Gene name: 12
Sequence domains: Pectate lyase superfamily protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CO 2 x CO
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: R32
Unit cell:
a: 89.741Å b: 89.741Å c: 584.795Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.222
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Host receptors for bacteriophage adsorption.
Bertozzi Silva et al. (2016)
10 more

2 mentions without citation

Crystallographic insights into the autocatalytic assembly mechanism of a bacteriophage tail spike.
Xiang et al. (2009)
1 more