› 3gk3

X-ray diffraction
2.1Å resolution

Function and Biology Details

Reaction catalysed: 
(R)-3-hydroxyacyl-CoA + NADP(+) = 3-oxoacyl-CoA + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174634 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetoacetyl-CoA reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 269 amino acids
Theoretical weight: 28.78 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JJT1 (Residues: 1-248; Coverage: 100%)
Gene names: BURPS1710b_A1014, phbB-2
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 70.57Å b: 102.92Å c: 137.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.277
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The design and analysis of transposon insertion sequencing experiments.
Chao et al. (2016)
10 more

1 mention without citation

Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh et al. (2013)