› 3gh4

X-ray diffraction
1.8Å resolution

Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12

Released:
DOI: 10.2210/pdb3gh4/pdb
PDB entry 3gh4 coloured by chain, front view.
PDB entry 3gh4 coloured by chain, side view.
PDB entry 3gh4 coloured by chain, top view.
Source organism: Paenibacillus sp.
Primary publication:
Molecular cloning and crystal structural analysis of a novel beta-N-acetylhexosaminidase from Paenibacillus sp. TS12 capable of degrading glycosphingolipids.
Sumida T, Ishii R, Yanagisawa T, Yokoyama S, Ito M
J Mol Biol 392 87-99 (2009)
PMID: 19524595

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112200 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-N-acetylhexosaminidase Chain: A
Molecule details ›
Chain: A
Length: 525 amino acids
Theoretical weight: 57.57 KDa
Source organism: Paenibacillus sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0VX21 (Residues: 1-505; Coverage: 100%)
Gene name: Hex1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2221
Unit cell:
a: 95.615Å b: 101.981Å c: 107.622Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.136 0.172
Expression system: Escherichia coli BL21(DE3)

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Citations

19 citation in other articles

Structural determinants of an insect beta-N-Acetyl-D-hexosaminidase specialized as a chitinolytic enzyme.
Liu et al. (2011)
18 more

3 mentions without citation

Probing the Catalytic Mechanism of Vibrio harveyi GH20 β-N-Acetylglucosaminidase by Chemical Rescue.
Meekrathok et al. (2016)
2 more