PDBe 3gf0

X-ray diffraction
2.62Å resolution

Bifunctional dCTP deaminase-dUTPase mutant enzyme variant E145Q from Methanocaldococcus jannaschii in complex with pyrophosphate and magnesium

Released:
Entry authors: Siggaard JHB, Johansson E, Vognsen T, Helt SS, Harris P, Willemoes M

Function and Biology Details

Reaction catalysed:
dCTP + 2 H(2)O = dUMP + diphosphate + NH(3). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dCTP deaminase, dUMP-forming Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.46 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57872 (Residues: 1-204; Coverage: 100%)
Gene names: MJ0430, dcd
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I432
Unit cell:
a: 171.569Å b: 171.569Å c: 171.569Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.229
Expression system: Escherichia coli