PDBe 3g9j

X-ray diffraction
2.32Å resolution

GR DNA binding domain:Pal, 18bp complex-36

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Glucocorticoid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 9.96 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06536 (Residues: 440-525; Coverage: 11%)
Gene names: Grl, Nr3c1
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*AP*AP*AP*TP*GP*TP*TP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*TP*TP*TP*TP*GP*TP*TP*CP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.48 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 39.069Å b: 102.096Å c: 112.076Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.184 0.226
Expression systems:
  • Escherichia coli
  • Not provided