PDBe 3g6y

X-ray diffraction
2.1Å resolution

Ternary complex of DNA Polymerase iota:DNA:dTTP with an abasic site at the templating position

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase iota Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 47.03 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9UNA4 (Residues: 26-445; Coverage: 57%)
Gene names: POLI, RAD30B
Sequence domains: impB/mucB/samB family
Structure domains:
Primer DNA strand Chain: P
Molecule details ›
Chain: P
Length: 7 nucleotides
Theoretical weight: 2.09 KDa
Source organism: Synthetic construct
Expression system: Not provided
Template DNA strand Chain: T
Molecule details ›
Chain: T
Length: 11 nucleotides
Theoretical weight: 3.21 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P6522
Unit cell:
a: 98.114Å b: 98.114Å c: 202.823Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.22 0.253
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided