› 3fyg

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF TETRADECA-(3-FLUOROTYROSYL)-GLUTATHIONE S-TRANSFERASE

Released:
DOI: 10.2210/pdb3fyg/pdb
PDB entry 3fyg coloured by chain, front view.
PDB entry 3fyg coloured by chain, side view.
PDB entry 3fyg coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Conformational changes in the crystal structure of rat glutathione transferase M1-1 with global substitution of 3-fluorotyrosine for tyrosine.
Xiao G, Parsons JF, Tesh K, Armstrong RN, Gilliland GL
J Mol Biol 281 323-39 (1998)
PMID: 9698551

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Mu 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 26.05 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04905 (Residues: 2-218; Coverage: 100%)
Gene name: Gstm1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GPR 2 x GPR
No bound ligands
1 modified residue:
Ligand YOF 26 x YOF

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 85.43Å b: 88.42Å c: 57.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 not available not available
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Challenges in enzyme mechanism and energetics.
Kraut et al. (2003)
3 more

1 mention without citation

Structural basis of Focal Adhesion Kinase activation on lipid membranes.
Acebrón et al. (2020)