PDBe 3flo

X-ray diffraction
2.5Å resolution

Crystal structure of the carboxyl-terminal domain of yeast DNA polymerase alpha in complex with its B subunit

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA polymerase alpha subunit B Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 460 amino acids
Theoretical weight: 51.96 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38121 (Residues: 246-705; Coverage: 65%)
Gene names: POL12, YBL035C, YBL0414
Sequence domains: DNA polymerase alpha/epsilon subunit B
DNA polymerase alpha catalytic subunit A Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 3 amino acids
Theoretical weight: 273 Da
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
DNA polymerase alpha catalytic subunit A Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 206 amino acids
Theoretical weight: 23.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P13382 (Residues: 1263-1468; Coverage: 14%)
Gene names: CDC17, N2181, POL1, YNL102W
Sequence domains: DNA Polymerase alpha zinc finger

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 85.411Å b: 142.633Å c: 175.255Å
α: 90° β: 102.33° γ: 90°
R-values:
R R work R free
0.192 0.191 0.218
Expression system: Escherichia coli