› 3fhr

X-ray diffraction
1.9Å resolution

High resolution crystal structure of mitogen-activated protein kinase-activated protein kinase 3 (MK3)-inhibitor complex

Released:
DOI: 10.2210/pdb3fhr/pdb
PDB entry 3fhr coloured by chain, front view.
PDB entry 3fhr coloured by chain, side view.
PDB entry 3fhr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution crystal structure of human Mapkap kinase 3 in complex with a high affinity ligand.
Cheng R, Felicetti B, Palan S, Toogood-Johnson I, Scheich C, Barker J, Whittaker M, Hesterkamp T
Protein Sci 19 168-73 (2010)
PMID: 19937655

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MAP kinase-activated protein kinase 3 Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 38.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16644 (Residues: 33-349; Coverage: 83%)
Gene name: MAPKAPK3
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand P4O 1 x P4O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 84.688Å b: 74.832Å c: 60.664Å
α: 90° β: 107.34° γ: 90°
R-values:
R R work R free
0.228 0.226 0.267
Expression system: Escherichia coli

Quick links

Citations

3 review citations

MAPKAPK2: the master regulator of RNA-binding proteins modulates transcript stability and tumor progression.
Soni et al. (2019)
2 more

10 mentions without citation

High-resolution crystal structure of human Mapkap kinase 3 in complex with a high affinity ligand.
Cheng et al. (2010)
9 more