PDBe 3fhi

X-ray diffraction
2Å resolution

Crystal structure of a complex between the catalytic and regulatory (RI{alpha}) subunits of PKA


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Chain: B
Molecule details ›
Chain: B
Length: 154 amino acids
Theoretical weight: 17.34 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
  • Canonical: P00514 (Residues: 92-245; Coverage: 41%)
Gene name: PRKAR1A
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 54.453Å b: 93.017Å c: 122.043Å
α: 90° β: 90° γ: 90°
R R work R free
0.199 0.198 0.226
Expression system: Escherichia coli