PDBe 3fgp

X-ray diffraction
2.05Å resolution

2.05 a Crystal Structure of CysM from Mycobacterium Tuberculosis - Open and Closed Conformations

Released:

Function and Biology Details

Reaction catalysed:
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH(2)-C(O)-S-L-cysteine + phosphate. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-phosphoserine sulfhydrylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 34.95 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WP53 (Residues: 1-323; Coverage: 100%)
Gene names: MTCY130.21, Rv1336, cysM
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 74.933Å b: 89.142Å c: 99.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.247
Expression system: Escherichia coli BL21(DE3)