› 3fc2

X-ray diffraction
2.45Å resolution

PLK1 in complex with BI6727

Released:
DOI: 10.2210/pdb3fc2/pdb
PDB entry 3fc2 coloured by chain, front view.
PDB entry 3fc2 coloured by chain, side view.
PDB entry 3fc2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
BI 6727, a Polo-like kinase inhibitor with improved pharmacokinetic profile and broad antitumor activity.
Rudolph D, Steegmaier M, Hoffmann M, Grauert M, Baum A, Quant J, Haslinger C, Garin-Chesa P, Adolf GR
Clin Cancer Res 15 3094-102 (2009)
PMID: 19383823

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156787 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK1 Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 37.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53350 (Residues: 13-345; Coverage: 55%)
Gene names: PLK, PLK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand IBI 1 x IBI
Ligand EDO 4 x EDO
Ligand ACT 2 x ACT
Ligand PEG 1 x PEG
Ligand ZN 1 x ZN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3221
Unit cell:
a: 66.616Å b: 66.616Å c: 153.969Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.183 0.226
Expression system: Spodoptera frugiperda

Quick links

Citations

59 review citations

Cell cycle proteins as promising targets in cancer therapy.
Otto et al. (2017)
58 more

9 mentions without citation

Recent Advances and New Strategies in Targeting Plk1 for Anticancer Therapy.
Lee et al. (2015)
8 more