PDBe 3f75

X-ray diffraction
1.99Å resolution

Activated Toxoplasma gondii cathepsin L (TgCPL) in complex with its propeptide

Released:

Function and Biology Details

Reaction catalysed:
Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cathepsin L Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 24.43 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DMN0 (Residues: 199-422; Coverage: 53%)
Gene names: CPL, TgIb.2320c
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases
Cathepsin L Chain: P
Molecule details ›
Chain: P
Length: 106 amino acids
Theoretical weight: 12.79 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DMN0 (Residues: 105-198; Coverage: 22%)
Gene names: CPL, TgIb.2320c
Sequence domains: Cathepsin propeptide inhibitor domain (I29)

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P43212
Unit cell:
a: 65.621Å b: 65.621Å c: 149.772Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.206
Expression system: Escherichia coli