PDBe 3f2r

X-ray diffraction
2.35Å resolution

Crystal structure of human choline kinase alpha in complex with hemicholinium-3

Released:
Source organism: Homo sapiens
Entry authors: Hong B, Tempel W, Rabeh WM, MacKenzie F, Arrowsmith CH, Edwards AM, Bountra C, Weigelt J, Bochkarev A, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed:
ATP + choline = ADP + phosphocholine. 
ATP + ethanolamine = ADP + O-phosphoethanolamine. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline kinase alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 46.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35790 (Residues: 75-457; Coverage: 84%)
Gene names: CHK, CHKA, CKI
Sequence domains: Choline/ethanolamine kinase
Structure domains: Aminoglycoside 3'-phosphotransferase; Chain: A, domain 2

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Rigaku FRE
Spacegroup: P212121
Unit cell:
a: 55.553Å b: 119.581Å c: 131.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.275
Expression system: Escherichia coli