› 3f1v

X-ray diffraction
1.77Å resolution

E. coli Beta Sliding Clamp, 148-153 Ala Mutant

Released:
DOI: 10.2210/pdb3f1v/pdb
PDB entry 3f1v coloured by chain, front view.
PDB entry 3f1v coloured by chain, side view.
PDB entry 3f1v coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Sliding clamp-DNA interactions are required for viability and contribute to DNA polymerase management in Escherichia coli.
Heltzel JM, Scouten Ponticelli SK, Sanders LH, Duzen JM, Cody V, Pace J, Snell EH, Sutton MD
J Mol Biol 387 74-91 (2009)
PMID: 19361435

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 40.35 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
Structure domains: DNA Polymerase III, subunit A, domain 2

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P1
Unit cell:
a: 40.838Å b: 64.471Å c: 72.111Å
α: 73.83° β: 82.72° γ: 83.76°
R-values:
R R work R free
0.22 0.215 0.282
Expression system: Escherichia coli

Quick links

Citations

2 review citations

DNA replication fidelity in Escherichia coli: a multi-DNA polymerase affair.
Fijalkowska et al. (2012)
1 more

1 mention without citation

Sliding clamp-DNA interactions are required for viability and contribute to DNA polymerase management in Escherichia coli.
Heltzel et al. (2009)