PDBe 3ezq

X-ray diffraction
2.73Å resolution

Crystal Structure of the Fas/FADD Death Domain Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
FAS-associated death domain protein Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 122 amino acids
Theoretical weight: 13.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13158 (Residues: 93-208; Coverage: 56%)
Gene names: FADD, GIG3, MORT1
Sequence domains: Death domain
Structure domains: Death Domain, Fas
Tumor necrosis factor receptor superfamily member 6 Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 115 amino acids
Theoretical weight: 13.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25445 (Residues: 223-335; Coverage: 37%)
Gene names: APT1, FAS, FAS1, TNFRSF6
Sequence domains: Death domain
Structure domains: Death Domain, Fas

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P61
Unit cell:
a: 126.218Å b: 126.218Å c: 299.274Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.234 0.278
Expression system: Escherichia coli