› 3eqs

X-ray diffraction
1.65Å resolution

Crystal structure of human MDM2 in complex with a 12-mer peptide inhibitor

Released:
DOI: 10.2210/pdb3eqs/pdb
PDB entry 3eqs coloured by chain, front view.
PDB entry 3eqs coloured by chain, side view.
PDB entry 3eqs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for high-affinity peptide inhibition of p53 interactions with MDM2 and MDMX.
Pazgier M, Liu M, Zou G, Yuan W, Li C, Li C, Li J, Monbo J, Zella D, Tarasov SG, Lu W
Proc Natl Acad Sci U S A 106 4665-70 (2009)
PMID: 19255450

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163516 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase Mdm2 Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 10.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q00987 (Residues: 25-109; Coverage: 17%)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain
12-mer peptide inhibitor Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.43 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GAI 1 x GAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 71.517Å b: 43.274Å c: 35.036Å
α: 90° β: 116.51° γ: 90°
R-values:
R R work R free
0.156 0.154 0.193
Expression system: Not provided

Quick links

Citations

46 review citations

MDM2, MDMX and p53 in oncogenesis and cancer therapy.
Wade et al. (2013)
45 more

27 mentions without citation

Targeting p53-MDM2-MDMX loop for cancer therapy.
Zhang et al. (2014)
26 more