› 3eoq

X-ray diffraction
2.29Å resolution

The crystal structure of putative zinc protease beta-subunit from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb3eoq/pdb
PDB entry 3eoq coloured by chain, front view.
PDB entry 3eoq coloured by chain, side view.
PDB entry 3eoq coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structure of TTHA1264, a putative M16-family zinc peptidase from Thermus thermophilus HB8 that is homologous to the beta subunit of mitochondrial processing peptidase.
Ohtsuka J, Ichihara Y, Ebihara A, Nagata K, Tanokura M
Proteins 75 774-80 (2009)
PMID: 19241474

Function and Biology Details

Reaction catalysed: 
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177977 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zinc protease Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 46.06 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SIV0 (Residues: 1-406; Coverage: 100%)
Gene name: TTHA1264
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 70.34Å b: 94.202Å c: 134.454Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.237
Expression system: Escherichia coli

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Citations

8 citation in other articles

Conformational states and recognition of amyloidogenic peptides of human insulin-degrading enzyme.
McCord et al. (2013)
7 more

2 mentions without citation

Crystal Structure and Function of PqqF Protein in the Pyrroloquinoline Quinone Biosynthetic Pathway.
Wei et al. (2016)
1 more