› 3enm

X-ray diffraction
2.35Å resolution

The structure of the MAP2K MEK6 reveals an autoinhibitory dimer

Released:
DOI: 10.2210/pdb3enm/pdb
PDB entry 3enm coloured by chain, front view.
PDB entry 3enm coloured by chain, side view.
PDB entry 3enm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the MAP2K MEK6 reveals an autoinhibitory dimer.
Min X, Akella R, He H, Humphreys JM, Tsutakawa SE, Lee SJ, Tainer JA, Cobb MH, Goldsmith EJ
Structure 17 96-104 (2009)
PMID: 19141286

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 316 amino acids
Theoretical weight: 36.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52564 (Residues: 45-332; Coverage: 86%)
Gene names: MAP2K6, MEK6, MKK6, PRKMK6, SKK3
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 52 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 122.663Å b: 122.663Å c: 195.54Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.212 0.269
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Mechanisms and functions of p38 MAPK signalling.
Cuadrado et al. (2010)
3 more

3 mentions without citation

The structure of the MAP2K MEK6 reveals an autoinhibitory dimer.
Min et al. (2009)
2 more