PDBe 3eim

X-ray diffraction
1.88Å resolution

Metal exchange in Thermolysin

Released:
Source organism: Bacillus thermoproteolyticus
Entry authors: Englert L, Heine A, Klebe G

Function and Biology Details

Reaction catalysed:
Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermolysin Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.36 KDa
Source organism: Bacillus thermoproteolyticus
UniProt:
  • Canonical: P00800 (Residues: 233-548; Coverage: 61%)
Gene name: npr
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P6122
Unit cell:
a: 92.71Å b: 92.71Å c: 128.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.252